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Part:BBa_K415151:Experience
Designed by: Shirley Galbiati Group: iGEM10_MIT (2010-10-24)
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Applications of BBa_K415151
Assessing p8-GR1 incorporation into phage coat
We transformed XL1-Blue cells with BBa_K415151, grew cells up without induction and infected with M13K07 helper phage. After 5 hours, the phage was purified, denatured, and western blotted with an anti-HA antibody.
The purple band at 13 kD represents the p8-GR1 fusion, and is evidence of the fusion protein's incorporation into the phage coat.
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