Regulatory
Part:BBa_K415511
Designed by: Joy Jiao, Laura Deming, Adrian Slusarczyk Group: iGEM10_MIT (2010-10-27)
pPAI2 L4R1 MammoBlock Entry Vector
L4R1 MammoBlock Promoter Vector. Contains plasminogen activator inhibitor 2 (PAI-2) promoter, shown to increase transcriptional activity in mammalian cells in response to radial and circumferential cellular strain on a cell monolayer
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 6
Illegal XbaI site found at 145
Illegal SpeI site found at 55 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 6
Illegal SpeI site found at 55 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 6
Illegal BglII site found at 1082
Illegal BglII site found at 1402 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 6
Illegal XbaI site found at 145
Illegal SpeI site found at 55 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 6
Illegal XbaI site found at 145
Illegal SpeI site found at 55 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1313
Illegal BsaI site found at 1578
Illegal BsaI.rc site found at 674
Illegal SapI.rc site found at 1016
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Categories
Parameters
None |