Measurement

Part:BBa_K313021

Designed by: Ryosuke Kamei, Ryo Kariyazono, Kosuke Uekusa   Group: iGEM10_UT-Tokyo   (2010-10-23)
Revision as of 08:01, 27 October 2010 by Pokeo (Talk | contribs)

terminator leakiness assay device

This is a device that aims to measure the leakiness of a terminator BBa_B0017.

If RNA polymerase transcribes the coding region of Cre regardless of the terminator, Cre is expressed from this part.

With our cre recombinase assay device such as BBa_K313016 or BBa_K313009, you would be able to detect the quantity of Cre and assess the leakiness of the terminator.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 479
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 165
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None