Coding

Part:BBa_K399002:Design

Designed by: Alyssa Henning and Malinka Walaliyadde   Group: iGEM10_Cornell   (2010-10-27)
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mTangerine


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 340
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 340
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mTangerine contains a PstI site. To make this part conform with RFC 10, we suggest digesting this BioBrick at EcoRI and XbaI. Digest the upstream sequence at EcoRI and SpeI.


Source

The coding sequence was on a pET28a plasmid bought from Invitrogen by the DeLisa lab at Cornell University.

References