Coding

Part:BBa_K399001:Design

Designed by: Alyssa Henning and Malinka Walaliyadde   Group: iGEM10_Cornell   (2010-10-26)
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mStrawberry


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 352
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 352
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 352
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 352
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

mStrawberry contains a PstI site. To make this part conform with RFC 10, we suggest digesting this BioBrick at EcoRI and XbaI. Digest the upstream sequence at EcoRI and Spe1.


Source

The coding sequence was on a pET28a plasmid bought from Invitrogen by the DeLisa lab at Cornell University.

References