Reporter

Part:BBa_K341002:Design

Designed by: Sheng WANG   Group: iGEM10_Tsinghua   (2010-10-20)
Revision as of 02:39, 28 October 2010 by Liteng07 (Talk | contribs) (→‎References)

promoter+tet-SDS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1147
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

1-25： LP1

26-43： I-scel

50-80：Plac

115-1320： Tet

1321-1338：I-Scel

1339-1363： LP2

Source

from vecter ptks-cs

References

Thomas E. Kuhlman and Edward C. Cox:Site-specific chromosomal integration of large synthetic constructs,Nucleic Acids Research, 2009, 1–10