Project

Part:BBa_K404208

Designed by: Freiburg Bioware 2010   Group: iGEM10_Freiburg_Bioware   (2010-08-28)
Revision as of 21:47, 26 October 2010 by Patrick (Talk | contribs) (→‎Usage and Biology)

ViralBrick-587-Beta Lactamase (BLA)

Freiburg10 ViralBrick-logo-587-BLA.png

Usage and Biology

ß-Lactamase (BLA) is an enzyme produced by some bacteria that cleaves the ß-lactam ring of various antibiotics such as penicillin, rendering them inactive. Because the peptides' N- and C-termini are in close proximity to each other, we created a ViralBrick containing BLA to see if capsid assembly still takes place with such a large insert in place. Assembled capsids can easily be detected by a beta-lactamase assay.







Restriction sites

Restriction site Enzyme Recognition sequence Activity Temp.
upstream 587 [http://www.neb.com/nebecomm/products/productR3136.asp BamHI-HF] Freiburg10 recognition site BamHI-HF.Gif 100;50;10;100 37°C
downstream 587 [http://www.neb.com/nebecomm/products/productR3151.asp PvuII-HF] Freiburg10 recognition site PvuII-HF.Gif 0;25;0;100 37°C

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 10
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote/human
//viral_vectors
//viral_vectors/aav
//viral_vectors/aav/miscellaneous
Parameters
None