Coding
RFP-AB-Bfu

Part:BBa_K349005

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-20)
Revision as of 02:38, 27 October 2010 by AlinaP (Talk | contribs)

RFP-AB-BfuAI (for construction of AB BioBytes 2.0)

Base plasmid v.2 for the construction of parts compatible with the BioBytes 2.0 assembly method. The RFP cassette can be removed using the BfuAI enzyme and a new AB ORF style may be inserted into the plasmid allowing for creation of the parts for the BioBytes 2.0 assembly method.

Usage and Biology

The base plasmids v.2 provide a method of creating parts compatible with the BioByte 2.0 assembly method. The RFP coding device provides a selective method for finding plasmids with your favorite part inserted.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
n/aRFP-AB-BfuAI (for construction of AB BioBytes 2.0)