Project

Part:BBa_K404234

Designed by: Freiburg Bioware 2010   Group: iGEM10_Freiburg_Bioware   (2010-08-28)
Revision as of 19:49, 26 October 2010 by Achim (Talk | contribs)

[AAV2]-Rep-VP123_P5-TATAless (ViralBrick-587KO-Z34C-Spacer)
The Z34C antibody binding motif including a knockout of the natural HSPG tropism & Spacer, inserted into the 587 loop of [AAV2]-Rep-VP123_p5-TATAless

Freiburg10 Rep-VP123 P5-TATA-less 587KO-Z34C-Spacer.png

Usage and Biology

The Z34C antibody binding motif is a 34 amino acids long fragment from the a minimized fragment of the Z Domain of Staphylococcal Protein A. It specifically binds to the Fc-Domain of antibodies. If inserted into AAV capsids, it can bind antibodies against a specific antigen, allowing differential targeting without the need for further genetic engineering.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 3611
    Illegal XhoI site found at 1913
    Illegal XhoI site found at 2099
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 4254
    Illegal BsaI site found at 4436
    Illegal BsaI site found at 4473
    Illegal SapI site found at 3048


[edit]
Categories
//chassis/eukaryote/human
//viral_vectors
//viral_vectors/aav
//viral_vectors/aav/capsid_coding
Parameters
None