Signalling

Part:BBa_K354001:Design

Designed by: Michelle Robinson   Group: iGEM10_WITS-South_Africa   (2010-10-19)
Revision as of 18:44, 19 October 2010 by Registry (Talk | contribs)

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PlcR-PapR - Gram+ve Quorum Peptide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence recognised by the SecA pathway had to be included in the synthesised sequence so as to ensure that the fusion peptide is adequately exported fro the cell. A ribozyme binding site (RBS) was also included in the final sequence. Furthermore, the sequence was codon optimized for Lactobacillus gasseri.


Source

This sequence was adapted from the naturally-occurring fusion peptide found in the Bacillus cereus genome.

References