Part:BBa_K395705

crtW (beta-carotene ketolase) with rbs

CrtW (beta-carotene ketolase) from Brevundimonas sp. strain SD212 (MBIC 03018)Accession: AB377271.

This is the enzyme of the carotenoid biosynthesis pathway. CrtW converts zeaxanthin to astaxanthin, the final metabolite of the pathway.

Fig. 2-1-11-2 astaxanthin pellet and plate.
This work is done by Yumiko Kinoshita

Fig. 2-1-11-2 astaxanthin pellet and plate.
This work is done by Yumiko Kinoshita

Fig. 2-1-12-2 astaxanthin extract.
This work is done by Yumiko Kinoshita

figure2.
This work is done by Yumiko Kinoshita

Fig. 2-1-1-3.TLC.jpg.
This work is done by Yumiko Kinoshita

In order to confirm that BBa_K395705 is worked, we construct a BioBrick part (BBa_K395706), which contains this part under BBa_K395702. We synthesized astaxanthin by BBa_K395706, when add beta-carotene as a substrate. Thus we confirmed that BBa_395705 is working. Characterization of BBa_K395706 has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 632
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 715
  Illegal SapI site found at 542
  Illegal SapI site found at 554