Generator

Part:BBa_K346010

Designed by: Qianzhu Wu   Group: iGEM10_Peking   (2010-10-18)
Revision as of 15:01, 22 October 2010 by Wqz (Talk | contribs)

Constutitive promoter(BBa_J23108)+RBS+MerR(BBa_K346001)

This part is composed of constutitive promoter BBa_J23108 and transcription factor MerR. It can regulate PmerT to represent a dose-response manner to response to Hg(II) concentration. We used the structure along with PmerT-RBS-GFP to see under the constant MerR concentration level how GFP expression vary according to the Hg(II) concentration.

3.jpg



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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