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Part:BBa_K395400:Experience

Designed by: Mitsuhiko Odera   Group: iGEM10_Tokyo_Tech   (2010-10-05)
Revision as of 12:50, 27 October 2010 by ODDY (Talk | contribs)

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Applications of BBa_K395400

LacIM1 shows weaker repression than LacIWT because it has a lower affinity to lac promoter [1]. Though this part was registered by USTC(2008), it was not well characterized in their wiki. Therefore, we characterized this part.

We confirmed that lacIM1 shows weaker repression than LacIWT.(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])
We ligated BBa_K395400 together with Pbad/araC promoter. Then, we used it together with BBa_I7106(lacI+pL-RBS-GFP) on pSB3K3 (Fig. 3-2-1). Fluorescence was measured, and the result is shown in Fig. 3-2-2.


Figure3-2-1.
Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG. This work is done by Mitsuhiko Odera


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