Part:BBa_K361000

Rci site-specific recombinase

This part will prouce a 384-amino acid Rci site-specific recombinase used in shufflon system, which is a site-specific DNA recombination between two specific repeat sites as recognition.

After this recombinase is being expressed, it can alter the DNA sequence being inserted randomly with the specific repeat sites originally to become an unknown sequence.

This is a Wide type Rci recombinase. In the recombination activity study: Intramolecular inversion frequency = 0.062 Intramolecular deletion frequency = 0 (recombinase inactive on this function)

Intermolecular recombination = 0 (recombinase inactive on this function)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 362
  Illegal NgoMIV site found at 857
  
• 1000
  COMPATIBLE WITH RFC[1000]