Generator

Part:BBa_K398014

Designed by: Kira Schipper and Mathias Voges   Group: iGEM10_TU_Delft   (2010-10-07)
Revision as of 20:43, 20 October 2010 by Mvoges (Talk | contribs)

Alkane Hydroxylase System

The alkane hydroxylase system from Gordonia sp. TF6 facilitates the initial step of the degradation of C5-C13 alkanes as well as that of C5-C8 cycloalkanes. The cluster is formed by the genes for alkB2 (alkane 1-monooxygenase), rubA3 (rubredoxin), rubA4 (rubredoxin) and rubB (rubredoxin reductase). alkB2 is a non-haem diiron monooxygenase membrane protein. This monooxygenase oxidizes (cyclo)alkanes to the respective (cyclo)alkanols, requires two soluble electron-carrier proteins, rubredoxin (rubA3 & rubA4) and rubredoxin reductase (rubB).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None