Generator
Part:BBa_K398014
Designed by: Kira Schipper and Mathias Voges Group: iGEM10_TU_Delft (2010-10-07)
Alkane Hydroxylase System
The alkane hydroxylase system from Gordonia sp. TF6 facilitates the initial step of the degradation of C5-C13 alkanes as well as that of C5-C8 cycloalkanes. The cluster is formed by the genes for alkB2 (alkane 1-monooxygenase), rubA3 (rubredoxin), rubA4 (rubredoxin) and rubB (rubredoxin reductase). alkB2 is a non-haem diiron monooxygenase membrane protein. This monooxygenase oxidizes (cyclo)alkanes to the respective (cyclo)alkanols, requires two soluble electron-carrier proteins, rubredoxin (rubA3 & rubA4) and rubredoxin reductase (rubB).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |