Part:BBa_K389002

Mutated virG

This is the virG response regulator from the VirA/G receptor system. The virA/G receptor system is used by Agrobacterium tumefaciens to detect phenolic substances secreted by injured plants, e.g. acetosyringone. In presence of these substances, VirG is activated by the VirA receptor and induces the transcription of genes under the control of a vir promoter. Normally, VirG needs the rpoA RNA polymerase subunit from A. tumefaciens to work in Escherichia coli but this virG BioBrick is mutated, so it works with the rpoA subunit from E. coli. For this reason the point mutations G56V and I77V were brought into the gene (compare YC Jung et al., 2004). Because this BioBrick is synthesized (Mr.Gene GmbH), codon usage is optimized for E. coli and illegal restriction sites were removed. When you use this virG gene in a VirA/G signaling system you do not need BBa_K238010 anymore to get the system working in E. coli.

Usage and Biology

For a working VirA/G-receptor system, you also need the virA BioBrick BBa_K389001 and a vir promoter BBa_K389003. You can find an already assembled VirA/G-receptor system here: BBa_K389017.

Functionality

The functionality of this BioBrick were testet with the devices: BBa_K389015, BBa_K389016.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]