Part:BBa_K320014
S + P25 + P28 + Ori + Tet
Constitutive expression of P25 and P28 (driven by K320002) with an origin for Asaia and E. coli and a resiatance to Tetracycline .
This BioBricks makes the bacteria resitant to Tetracycline, contains an origin of replication for Asaia/E. coli and drives constitutive expression of the proteins P28 and P25.
The ookinete (plasmodium state in mosquito's gut) has been intensively studied by scientists, looking for an ideal transmission-blocking vaccine target. IGEM EPFL team's interest goes toward the ookinete surface membrane proteins P25 and P28. In fact, it has been shown that the binding of specific antibodies to these surface proteins was fatal to more than 99% of the parasites reference. Indeed, P25 and P28 seem to be essential to the ookinete survival, the crossing of the midgut epithelium and its transformation into an oocyst.
The team decided to design Asaia to express a soluble form of these 2 P-proteins. It could seems contra-intuitive to provide more of these proteins (by expression in Asaia) that are very important for the parasite survival, migration, and development. But our hypothesis is that adding a lot of these proteins in a soluble form will compete with the parasite surface protein and prevent the interaction necessary to its transmission. Because the functions of P25 and P28 are redundant, both proteins need to be inhibited or outcompeted to efficiently block the malaria transmission.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 62
Illegal NheI site found at 827 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2699
Illegal AgeI site found at 2859 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1537
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