Composite

Part:BBa_K389423

Designed by: Jonas Aretz   Group: iGEM10_Bielefeld-Germany   (2010-10-11)
Revision as of 16:06, 16 October 2010 by Jaretz (Talk | contribs)

VirA/G signaling system + sensitivity tuner + luciferase

This part combines a complete VirA/G signaling system with a sensitivity tuner before a luciferase gene under the control of a vir promoter. The sensitivity tuner enhances a signal from a promoter so it enhances the readout luciferase.

Usage and Biology

To see, how this BioBrick works, watch the following animation:

Animation of the functionality of the BioBricks BBa_K389421, BBa_K389422 and BBa_K389423.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 647
    Illegal NheI site found at 2581
    Illegal NheI site found at 2604
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1632
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 5029
    Illegal NgoMIV site found at 6373
    Illegal NgoMIV site found at 6394
    Illegal AgeI site found at 4552
    Illegal AgeI site found at 4664
    Illegal AgeI site found at 4866
    Illegal AgeI site found at 6097
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1768
    Illegal SapI.rc site found at 6279


[edit]
Categories
//cds/membrane/receptor
//chassis/prokaryote/ecoli
//classic/measurement/uncategorized
//function/reporter/light
Parameters
None