Generator
S+imm+ori+

Part:BBa_K320008:Design

Designed by: Perrudet Christian   Group: iGEM10_EPF_Lausanne   (2010-10-18)
Revision as of 14:07, 24 October 2010 by Perrudet (Talk | contribs) (→‎Source)

Constitutive expression of Immunotoxin in Asaia and resistance to Kanamycine


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 62
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 883
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2297
    Illegal AgeI site found at 2457
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1135


Design Notes

Ligation of both part quote below, which were ligation of BBa_K320001/BBa_K320002 and BBa_K320000/BBa_P1005.

Source

Both BioBrick we used were BBa_K320006 and BBa_K320004.

References