Generator
MpAAT1
Part:BBa_K395602:Design
Designed by: Toshitaka Matsubara Group: iGEM10_Tokyo_Tech (2010-10-04)
Apple fragrance generator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1765
Illegal NotI site found at 1666 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 195
Illegal BamHI site found at 1635
Illegal XhoI site found at 1675 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 205
Illegal AgeI site found at 1419 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
T7 promoter+lac operator+RBS+His tag+thirombin+S・tag+Factor Xa+MpAAT1+His tag+T7 terminator
Source
apple