Generator

Part:BBa_K395400

Designed by: Mitsuhiko Odera   Group: iGEM10_Tokyo_Tech   (2010-10-05)
Revision as of 05:34, 27 October 2010 by ODDY (Talk | contribs)

RBS-lacIM1-ter


LacIM1 shows weaker repression than LacIWT because it has a lower affinity to lac promoter [1]. LacIM1 (BBa_K082026) which is the key component in the band-detect network. (Fig. 3-2-1) [1]. Though this part was registered by USTC(2008), its registered sequence is incorrect and it was not well characterized in their wiki. Therefore, we registered this part as BBa_K395400.

Figure3-2-1.
Figure3-2-2. Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG. This work is done by Mitsuhiko Odera

We confirmed that lacIM1 shows weaker repression than LacIWT.

(→[http://2010.igem.org/Team:Tokyo_Tech/Project/wolf_coli/lacIM1 more information])


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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