Signalling

Part:BBa_K422001

Designed by: Katharina Zwicky   Group: iGEM10_ETHZ_Basel   (2010-10-14)
Revision as of 11:59, 19 October 2010 by Zwickyk (Talk | contribs)

Archeal light receptor fused to bacterial chemotaxis transducer

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 747
    Illegal AgeI site found at 840
  • 1000
    COMPATIBLE WITH RFC[1000]

Usage and Biology

Fusion of the Natronobacterium pharaonis Np seven-transmembrane retinylidene photoreceptor sensory rhodopsins II NpSRII and their cognate transducer HtrII to the cytoplasmic domain of the chemotaxis transducer EcTsr of Escherichia coli.

Design

De novo Synthesis by GeneArt. Codon optimized.

References

[1] Jung, Spudich, Trivedi and Spudich: An archaeal photosignal-transducing module mediates phototaxis in Escherichia coli. Journal of bacteriology. 2001; 21.

[edit]
Categories
Parameters
excitation500 nm