Part:BBa_K427008
Ter PBadweak Mu Mor Ter PM GFP cassette
This part was used for the characterization of the MuMor sensitivity tuner. It is composed of seven biobricks starting with Ter PBadweak, which is the part created by British Columbia team in 2007 starts with a terminator and a promoter that respond to high concentrations of Arabinose. It is followed by sequence coding for Mor protein which inter activates the Pm promoter and this enhance the PoPS activity. Then is followed by the GFP cassette sequence a biobrick.
This part has no special safety considerations. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 228
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 168
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1501
.compatibility div ul,
.compatibility div li {
display: inline;
}
.compatibility div li {
position: relative;
padding-top: 2px;
padding-left:4px;
padding-right:3px;
margin-right:2px;
margin-bottom: 5px;
}
.compatibility div .box {
top: 35px;
width: 200px;
left: 0px;
}
.compatibility div .box white {
border: 1px solid gray;
background-color: white;
}
.compatibility div .box red {
border: 1px solid #dd6666;
background-color: #ffcccc;
background-image: url('https://parts.igem.org/images/red not/red box.png');
background-repeat: none;
}
.compatibility div .box green {
border: 1px solid #44ee44;
background-color: #aaffaa;
}
Assembly Compatibility:
10COMPATIBLE WITH RFC[10]
12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 228
21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 168
23COMPATIBLE WITH RFC[23]
25COMPATIBLE WITH RFC[25]
1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1501
show sequence and features system( 'seq features div', 'BBa K427008', '0');
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 228
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 168
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1501
.compatibility div li { display: inline; } .compatibility div li { position: relative; padding-top: 2px; padding-left:4px; padding-right:3px; margin-right:2px; margin-bottom: 5px; } .compatibility div .box { top: 35px; width: 200px; left: 0px; } .compatibility div .box white { border: 1px solid gray; background-color: white; } .compatibility div .box red { border: 1px solid #dd6666; background-color: #ffcccc; background-image: url('https://parts.igem.org/images/red not/red box.png'); background-repeat: none; } .compatibility div .box green { border: 1px solid #44ee44; background-color: #aaffaa; }
Assembly Compatibility:
10COMPATIBLE WITH RFC[10] 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 228 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 168 23COMPATIBLE WITH RFC[23] 25COMPATIBLE WITH RFC[25] 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1501
show sequence and features system( 'seq features div', 'BBa K427008', '0');References
BBa_F2620 Parts Registry entry with characterisation.
Kumaraswami, M., & Howe, M. a.-W. (2004). Crystal Structure of the Mor Protein of Bacteriophage Mu, a Member of the Mor/C Family of Transcription Activators. The Journal of biological Chemistry, 16581–16590.
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