Composite

Part:BBa_K313036

Designed by: Tomoka Ao, Ryosuke Kamei, Ryo Kariyazono, Masahiko Shimizu, Kosuke Uekusa, Mami Nakatake   Group: iGEM10_UT-Tokyo   (2010-10-26)
Revision as of 08:03, 26 October 2010 by Registry (Talk | contribs)

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MS2 phage cDNA (loading sequence and replicase-deficient)

Loading sequence deficient MS2 phage cDNA. Virus transformed from this part carry out mRNA with specific loading sequence(19bp) and infect another E.coli. In the infected E.coli, translation of mRNA occur.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
    Illegal BamHI site found at 2057
    Illegal XhoI site found at 1048
    Illegal XhoI site found at 3214
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 103
    Illegal EcoRI site found at 1628
    Illegal NgoMIV site found at 706
    Illegal NgoMIV site found at 1733
    Illegal NgoMIV site found at 2437
    Illegal NgoMIV site found at 3309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1665
    Illegal BsaI.rc site found at 163
    Illegal BsaI.rc site found at 2257


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Categories
Parameters
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