Reporter
Strong+CFP

Part:BBa_K320005:Experience

Designed by: Perrudet Christian   Group: iGEM10_EPF_Lausanne   (2010-10-18)
Revision as of 09:57, 27 October 2010 by Perrudet (Talk | contribs) (→‎Applications of BBa_K320005)

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Applications of BBa_K320005

We used this BioBrick to characterize the promoter we want to use in Asaia BBa_K320002. By monitoring the CFP expression in time we could measure the activity of the promoter. Moreover to see if the presence of the CFP hindrate the growth of the clones we measured the growth curve.
Both measurements were done contemporaneously in E.coli DH5a over 970 minutes (16 hours) at 37°C and the results are averaged over 6 samples.
The control for the autofluorescence and the growthcurve were done with a wildtype strain of DH5a. Unfortunately we can't compare our measurements with a well known promoter.
Further characterisation will focus on the measurement of the promoter's activity in Asaia.


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