Project

Part:BBa_K494003

Designed by: Haissi Cui, Tilman Flock, Sebastian Gude, Christoph Hartlmüller, Florian Praetorius, Jan Schüürmann, Tobi Wauer, Philipp Wortmann   Group: iGEM10_TU_Munich   (2010-10-25)
Revision as of 09:31, 27 October 2010 by Cui (Talk | contribs)

Exemplary insert for BBa_K494001 (His-Term)

This is a ready to use part consisting of our screening backbone BBa_K494001, a modified version of pSB1A10, the PBad Promotor BBa_I13453, the His Terminator and the reporter protein mCherry BBa_J06702 including RBS and double Terminator BBa_B0015.
This part belongs to the screening system for evaluation of individual switches constructed on the principle of bioLOGICS, in this case BBaK494003 may serve as a reference to compare results with those presented by the TU Munich 2010 iGEM Team. Together with BBaK494004 (Terminator plus small it is a setup to evaluate termination and antitermination efficiency of the His-Terminator.
Furthermore BBaK494003 can also be used as a reference in any other measurement concerning Terminator efficiency. The His-Terminator was found to be a strong terminator with allowed no detectable transcription of the following mCherry gene.
The His-Terminator (which does not exist as an individual BioBrick since there are enough thoroughly tested Terminators yet in the registry) is based on an attenuator stem loop in front of the His-operon of Salmonella enterica.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None