Coding
cinr

Part:BBa_C0077

Designed by: crackdots   Group: Antiquity   (2004-01-27)
Revision as of 01:49, 30 April 2006 by Registry (Talk | contribs)

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cinR activator from Rhizobium leguminosarum (+LVA)

In complex with O3-C14:1-HSL (made by BBa_C0076), CinR binds to the Cin promoter (BBa_R0077) and activates transcription.

Usage and Biology

In Rhizobium leguminosarum, cinR and cinI are forward-pointing, adjacent, and separate transcriptional elements. There is a short (<200bp) intergenic region between cinR and cinI. The cinR/intergenic/cinI structure is conserved for the cinRI locus in Rhizobium etli, and for the cerRI locus in Rhodobacter sphaeroides. The intergenic region is thought to contain a terminator for cinR, a promoter and RBS for cinI, and a dna-binding domain for cinR and its homologs, though a conserved structure has not yet been identified.

Sequence and Features


Barcodes are discontinued, but one was appended to the sequence of this part. Composite parts using this part will include the barcode. More ...

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 528
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

directionForward
functionAct
ligandsO3-C14:1-HSL
proteincinR
swisspro~ Q84HT2
tagLVA
[edit]
Categories
//cds
//cds/transcriptionalregulator/activator
//function/cellsignalling
//function/regulation/transcriptional
Parameters
directionForward
functionAct
ligandsO3-C14:1-HSL
proteincinR
swisspro~ Q84HT2
tagLVA