Translational_Unit

Part:BBa_K371005:Design

Designed by: Yang Zhang   Group: iGEM10_USTC   (2010-08-27)
Revision as of 08:26, 27 August 2010 by Registry (Talk | contribs)

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pduTUV(Propanediol utilization gene T+U+V)[RBS+pduT+RBS+pduU+RBS+pduV]


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 815
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Sequencing result shows that there are several silent mutation in pduT and pduU. PduV has some error mutation. We are still working on the function assay to certify whether the mutation pduV still function well.


Source

pduJK come from Citrobacter Freundii genenom(NBRC NO.12681)

References