Coding
Part:BBa_K346007:Design
Designed by: miao jing Group: iGEM10_Peking (2010-10-15)
Antigen 43
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 709
Illegal NgoMIV site found at 1933
Illegal NgoMIV site found at 2443
Illegal NgoMIV site found at 2464
Illegal AgeI site found at 1459
Illegal AgeI site found at 2203
Illegal AgeI site found at 2617
Illegal AgeI site found at 2859 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
We got the coding sequence of this gene and add a standard RBS(B0034) to replace the original RBS of this gene, which is regulated by other factors in the cell.
Source
K12 strain of E.Coli