Generator

Part:BBa_K395704

Designed by: Yumiko Kinoshita   Group: iGEM10_Tokyo_Tech   (2010-10-04)
Revision as of 01:55, 27 October 2010 by Kino (Talk | contribs)

zeaxanthin synthesis operon (pBad+rbs+crtZ+plac+rbs +crtEBIY )

This part contains all five genes required, CrtEBIYZ, to produce zeaxanthin.

It is able to control the expression of crtZ by arabinose.

Fig. 1.1.8.1 zeaxanthin pellet and plate
figure1

These parts contain crtZ (β-carotene hydroxylase) under inducible promoter control. This enzyme is responsible for the conversion of β-carotene into zeaxanthin. Our team designed some BioBricks to do this conversion. Characterization of these BioBricks has been performed in E. coli strain MG1655. (→[http://2010.igem.org/Team:Tokyo_Tech/Project/Apple_Reporter more information])


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
    Illegal BamHI site found at 3810
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 3346
    Illegal NgoMIV site found at 3476
    Illegal AgeI site found at 979
    Illegal AgeI site found at 2561
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


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Categories
Parameters
None