Coding
vioB-AB

Part:BBa_K349110

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-20)
Revision as of 04:59, 26 October 2010 by KarinaA (Talk | contribs)

vioB-AB (BioBytes 2.0)

From the Vio Operon. Used to produce a light green pigment from L-tyrosine. Depending on which parts of the operon are available a different colour will be produced. Please note this piece is naked meaning it does not contain a promoter, RBS, start or stop codon. The promoter and terminators are intended to be reconstituted through the adjoining linker Bytes attached through the BioByte Assembly Method v.2.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2529
    Illegal AgeI site found at 2725
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 3006


[edit]
Categories
Parameters
n/avioB-AB (BioBytes 2.0)