Coding
RFP-AB-Bsa
Part:BBa_K349001
Designed by: David Lloyd Group: iGEM10_Alberta (2010-10-20)
RFP-AB-BsaI (for construction of AB BioBytes 2.0)
Base plasmid v.2 for the construction of BioBytes-v2. The RFP cassette can be removed using the BsaI enzyme and a new AB ORF style part may be inserted into the plasmid for amplification.
Usage and Biology
This plasmid is used to create AB ORF style parts for the BioByte Assembly Method v.2.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 792
Illegal AgeI site found at 904 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI.rc site found at 1086
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Categories
Parameters
n/a | RFP-AB-BsaI (for construction of AB BioBytes 2.0) |