Coding

Part:BBa_K364319

Designed by: Lior Malka   Group: iGEM10_Debrecen-Hungary   (2010-10-08)
Revision as of 11:37, 26 October 2010 by Mrtwister (Talk | contribs)

Gal4 DBD

This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which encode for the enzymes used to convert galactose to glucose. This protein contains a fungal Zn(2)-Cys(6) binuclear cluster domain.

Our Gal4 is flanked by RFC 25 Prefix and Suffix, and thus can be used for assembly of in frame composite parts.

Picture of gel electrophoresis: Gal4 in pSB1C3 results a 441 bp long insert.
3D structure of Gal4 DBD

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 218
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 137


[edit]
Categories
Parameters
n/aGal4 DBD