Composite

Part:BBa_K374008:Experience

Designed by: Lisa Blanc Iversen, Maya Friis Kjaergaard, Annemi Jollmann, Anja Sander and Grzegorz Slodkowicz   Group: iGEM10_DTU-Denmark   (2010-10-20)
Revision as of 17:10, 26 October 2010 by MayaFriis (Talk | contribs) (→‎Biolector experiment)

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K374008

Biolector experiment

The biobrick was cloned into the pSB4A5 plasmid, and a GFP reporter gene was inserted downstream of it. The plasmid was transformed into E.coli (DH5 alpha) and measurements were done on this strain using a BioLector microreactor system. The BioLector is a microfermenting system that measures OD and fluorescence simultaneously. From this construct no GFP expression is expected as repression of the pR promoter is tight. As a reference the repressor was removed from the construct.

User Reviews

UNIQa992c3372d5b4c5b-partinfo-00000000-QINU UNIQa992c3372d5b4c5b-partinfo-00000001-QINU