Generator
B-carotene
Part:BBa_K343006
Designed by: Christian Kurtzhals Group: iGEM10_SDU-Denmark (2010-10-14)
Expresses B-carotene monooxygenase on a constitutive promotor
This generator expresses a beta-carotene 15,15'-monooxygenase,on a constitutive promotor, that catalyses retinal production from beta-carotene via the following reaction:
beta-carotene + O2 = 2 retinal
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 765
Illegal BamHI site found at 500
Illegal BamHI site found at 1757 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1361
Illegal BsaI site found at 1809
[edit]
Categories
Parameters
//cds/biosynthesis
//cds/enzyme
//chassis/prokaryote/ecoli
//classic/generator/prct
//classic/generator/uncategorized
//function/biosynthesis/isoprenoid
//cds/enzyme
//chassis/prokaryote/ecoli
//classic/generator/prct
//classic/generator/uncategorized
//function/biosynthesis/isoprenoid
function | Retinal Biosynthesis |
input_s | Beta-Carotene |
output | All-trans retinal |