Reporter

Part:BBa_K362065

Designed by: Fumihiko Takeuchi, Aya Iwaki, Akiko Kawashima , Yuki Fukuyama   Group: iGEM10_KIT-Kyoto   (2010-10-26)
Revision as of 11:00, 26 October 2010 by Registry (Talk | contribs)

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ahpC promoter - RBS - GFP - T-T - sufA promoter - RBS - RFP - T-T - dps promoter - RBS - CFP - T-T

This composite part has ahpC promoter(BBa_K362001), RBS(BBa_K121013), GFP(BBa_K121013), two terminators(BBa_K121013), sufA promoter(BBa_K362005), RBS(BBa_I13507), RFP(BBa_I13507), two terminators(BBa_I13507), dps promoter(BBa_K362002), RBS(BBa_E0420), CFP(BBa_E0420) and two terminators(BBa_E0420).

ahpC promoter,sufA promoter and dps promoter are controlled by OxyR protein, an oxidative stress resoponsive transcription facor.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 780
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 780
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1034
    Illegal BamHI site found at 1023
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 780
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 780
    Illegal AgeI site found at 3528
    Illegal AgeI site found at 3640
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1726


[edit]
Categories
Parameters
None