Composite

Part:BBa_K381001

Designed by: Katharine Coyte   Group: iGEM10_BCCS-Bristol   (2010-10-05)
Revision as of 10:49, 5 October 2010 by Registry (Talk | contribs)

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Nitrate reporter: PyeaR - GFP composite

Nitrate and Nitrite sensitive promoter PyeaR with a GFP coding device and strong RBS to create a nitrate-sensitive system which signals through expression of GFP.

Optimum use appears to be when sensing nitrate within a range of 0 - 20 mM

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 773


[edit]
Categories
//collections/immune_regulation/devices
Parameters
None