Part:BBa_K346033

T7p(BBa_I719005)+RBS(B0034)+DsbA+MBP(periplasm display of mercury binding peptide)

This part is a combination of T7promoter(BBa_I719005),RBS(B0034)and DsbA-MBP(periplasm display of mercury binding peptide)

Description

DsbA, a protein that can export its C-terminal fusion protein into periplasm more efficiently and faster than maltose binding protein, was used as the key component to express our Metal Binding Peptide periplasmically .

Figure 1: The structure of Dsba+MBP. This protein's function is to carry MBP to the periplasm and bind heavy metal there.

Figure 2: The expression of Dsba+MBP. We can see that the 40 kd band both in the SDS-PAGE and Western blotting result.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 529
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 529
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 317
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 529
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 529
  Illegal AgeI site found at 149
  
• 1000
  COMPATIBLE WITH RFC[1000]