Device
Part:BBa_K302012:Experience
Designed by: Rachel May Boyd, Phil Hall Group: iGEM10_Newcastle (2010-08-12)
Revision as of 02:26, 26 October 2010 by Swoodhouse (Talk | contribs) (→Applications of BBa_K302012)
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K302012
The filamentous cell part is repressed by LacI. The LacI repression is removed by induction with IPTG. Without LacI repression a higher proportion of the cells are filamentous. The filamentous cell phenotype is achieved by overexpressing yneA which is an indirect inhibitor of cell division.
| Graph1: |
|
| Graph 1 shows that overexpression (no Lac repression) of the yneA gene (ΔamyE:pSpac(hy)-oid::yneA) leads to a longer cell length compared with our control Bacillus subtilis 168. |
| See below: Bacillus subtilis 168 cells (left),Bacillus subtilis expressing yneA(centre) and Bacillus subtilis overexpressing yneA(right) |
  
|
| Graph2: |
|
| Graph 2 shows the percentage of cells at different lengths(μm)uninduced |
| See below: Bacillus subtilis 168 cells (left) and non-induced cells(right) |
|
| Graph3: |
|
| Graph 3 shows the percentage of cells at different lengths(μm)induced at 0.2mM IPTG |
| See below: Bacillus subtilis 168 cells (left) and cells induced at 0.2mM IPTG(right) |
|
| Graph4: |
|
| Graph 4 shows the percentage of cells at different lengths(μm)induced at 1mM IPTG |
| See below: Bacillus subtilis 168 cells (left) and cells induced at 1mM IPTG(right) |
|
Graphs 2,3 and 4 show a greater proportion of cells at a higher concentration of IPTG(1mM IPTG), compared with Bacillus subtilis 168 our control population.
User Reviews
UNIQ07bad1362baca7ef-partinfo-00000000-QINU UNIQ07bad1362baca7ef-partinfo-00000001-QINU