Device

Part:BBa_K302018:Design

Designed by: Steven Woodhouse, Alan Koh, Rachel May Boyd, Philip Hall   Group: iGEM10_Newcastle   (2010-08-18)
Revision as of 13:20, 18 August 2010 by Registry (Talk | contribs)

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Subtilin immunity


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 837
    Illegal BglII site found at 1585
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1852
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1132
    Illegal SapI site found at 1412
    Illegal SapI.rc site found at 2135


Design Notes

SpaI uses spoVG RBS (BBa_K143021), spaFEG uses native RBS (spaF and spaE RBS are in the coding sequence of preceding protein coding sequence). SpaG has had its TAG stop codon replaced with TAATAA.


Source

Gene Bank

References