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Coding
KanR-BA-Bs

Part:BBa_K349008

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-21)
Revision as of 23:38, 21 October 2010 by Registry (Talk | contribs)

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KanR-BA-BsaI (for construction of BA BioBytes 2.0)

BioByte Assembly Method-v2 testing plasmid containing the kanamycin resistance gene. Digestion with BsaI allows for the addition of any other ORF or part to the plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 984


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Categories
Parameters
n/aKanR-BA-BsaI (for construction of BA BioBytes 2.0)