Coding
KanR-AB-Bs

Part:BBa_K349007

Designed by: David Lloyd   Group: iGEM10_Alberta   (2010-10-21)
Revision as of 23:37, 21 October 2010 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


KanR-AB-BsaI (for construction of AB BioBytes 2.0)

BioByte Assembly Method-v2 testing plasmid containing the kanamycin resistance gene. Digestion with BsaI allows for the addition of any other ORF or part to the plasmid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 984


[edit]
Categories
Parameters
n/aKanR-AB-BsaI (for construction of AB BioBytes 2.0)