Coding

Part:BBa_K364317:Design

Designed by: Lior Malka   Group: iGEM10_Debrecen-Hungary   (2010-10-02)
Revision as of 20:22, 25 October 2010 by Lityupix (Talk | contribs) (→‎Design Notes)

NHR-31 LBD


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 229
    Illegal SapI.rc site found at 99


Design Notes

The construction of the LBD and pSB1C3 backbone have been done with RFC 25.

Source

C. elegans orphan nuclear receptor NHR-31. Arteficially synthetized Ligand Binding Domain of C. elegans NHR-31 nuclear receptor, placed into pSB1C3 carrier vector.

References

1. Hahn-Windgassen A, Van Gilst MR."The Caenorhabditis elegans HNF4alpha Homolog, NHR-31, mediates excretory tube growth and function through coordinate regulation of the vacuolar ATPase." [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2720251/?tool=pubmed PLoS Genet.2009 Jul;5(7):e1000553. Epub 2009 Jul 10.]