Regulatory
pBad/araC

Part:BBa_I0500

Designed by: Sri Kosuri   Group: Antiquity   (2003-12-05)
Revision as of 01:49, 30 April 2006 by Registry (Talk | contribs)

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Inducible pBad/araC promoter


-- No description --

Usage and Biology

When grown with 0.2% arabinose, promoter is weak-medium. [jb, 5/24/04] Part may not be compatible with MC4100 as cell line is araD 139.

PC and AraC are located on the complementary strand, reading right to left as written.

At least one registry stock contains a deletion of the C at base 1194. This is after the transcriptional start but before the translation start, so it may not be significant. Parts with this mutation have been qualitatively observed to function normally.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1205
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1144
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961

Functional Parameters

biology-NA-
controlaraC, arabinose
directionForward
n/aInducible pBad/araC promoter
negative_regulators-NA-
o_h-NA-
o_l-NA-
positive_regulators1
[edit]
Categories
//rnap/prokaryote/ecoli/sigma70
//direction/forward
//chassis/prokaryote/ecoli
//promoter
//regulation/positive
//classic/regulatory/other
Parameters
biology
controlaraC, arabinose
directionForward
n/aInducible pBad/araC promoter
negative_regulators
o_h
o_l
positive_regulators1