Coding
BMP2

Part:BBa_K415500

Designed by: Laura Deming, Joy Jiao, Adrian Slusarczyk   Group: iGEM10_MIT   (2010-10-24)
Revision as of 21:47, 24 October 2010 by Registry (Talk | contribs)

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BMP2 L1L2 MammoBlock Entry Vector

BBa_K415500 is an L1L2 Entry Vector for MammoBlock recombination cloning, containing the gene BMP2 (see below)

BMP2 (Bone Morphogenetic Protein 2) is a well estabished pro-osteogenic morphogen shown to induce osteogenesis in a variety of cell types in literature. Upon binding BMP2, the BMPRI receptor activates a downstream signaling cascade involving Smad proteins, which results in activation of the Runt-related transcription factor 2 (RUNX2). RUNX2 is the main transcription factor controlling osteoblast differentiation; transfection of RUNX2 induced osteoblastic differentiation of fibroblasts, which do not normally differentiate into osteoblasts.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1218
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1218
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1218
    Illegal BamHI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1218
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1218
    Illegal AgeI site found at 11
    Illegal AgeI site found at 313
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 218


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Categories
Parameters
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