Part:BBa_K320006:Experience
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
We tested expression of the immunotoxin in E.Coli (see [http://2010.igem.org/wiki/index.php?title=Team:EPF_Lausanne/Project/Materials_Methods Materials and Methods] for details). In a western blot analysis of whole cell lysates we could see bands corresponding to full length immunotoxin and possibly degraded fragments of the protein (see figure). The immunotoxin contains a PelB sequence that targets it for secretion into the periplasm. We concentrated the supernatant of both the immunotoxin and a control culture by running it through a filtering device with a 5 kDa cut-off. Running a western blot with these samples (see figure) we concluded that the immunotoxin was secreted as expected.
User Reviews
UNIQd5d0099afc2dcf95-partinfo-00000000-QINU UNIQd5d0099afc2dcf95-partinfo-00000001-QINU