Device
Part:BBa_K346005
Designed by: Junyi Jiao, Liang Donghai, Hu Yang&Teng Xin Group: iGEM10_Peking (2010-10-14)
Mercury (II) ions absorption device
This part contains three subparts,the dsba-mbp, the mbp and the lpp-ompa-mbp. Each subpart is driven by a T7promoter along with a rbs and terminator, which will express separately in the periplasm,the cytoplasm and the membrane to bind Hg(II).
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 529
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 529
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 317
Illegal BamHI site found at 1148
Illegal BamHI site found at 2111 - 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 529
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 529
Illegal AgeI site found at 149 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
n/a | Mercury (II) ions absorption device |