DNA
Ori

Part:BBa_K320000:Design

Designed by: Perrudet Christian   Group: iGEM10_EPF_Lausanne   (2010-10-17)
Revision as of 11:32, 24 October 2010 by Perrudet (Talk | contribs) (→‎Design Notes)

Asaia replication origin


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1160
    Illegal AgeI site found at 1320
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This has been obtain by PCR from a strand of Asaia containing a plasmid coding for GFP provided to us by the team of Prof. Guido Favia from Dipartimento di Medicina Sperimentale e Sanità Pubblica, Università degli Studi di Camerino, 62032 Camerino, Italy.

Source

PCR amplified from pHM2-GFP [http://www.pnas.org/content/104/21/9047.long [1]].

References

  1. [http://www.pnas.org/content/104/21/9047.long Bacteria of the genus Asaia stably associate with Anopheles stephensi, an Asian malarial mosquito vector]
  2. [http://aem.asm.org/cgi/content/abstract/68/5/2619 Cloning of Escherichia coli lacZ and lacY Genes and Their Expression in Gluconobacter oxydans and Acetobacter liquefaciens]