Reporter

Part:BBa_K325100

Designed by: Ben Reeve and Paul Masset   Group: iGEM10_Cambridge   (2010-09-15)
Revision as of 17:39, 15 September 2010 by Registry (Talk | contribs)

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EPIC Firefly Luciferase and LRE
P. Pyralis
(E. coli optimised)

This is a mutant luciferase that is very much brighter than wild-type. It is supplied in an operon with an enzyme (LRE) that regenerates its substrate. When supplied with luciferin and placed under a promoter it will emit light at a green wavelength. If D-cysteine is added the LRE will be able to regenerate the luciferin substrate.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2580
    Illegal BamHI site found at 948
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2284


[edit]
Categories
Parameters
None