Coding
Tse2

Part:BBa_K314200

Designed by: Matthew Coyne, Ingrid Swanson, Jesa Landis, Matthew Harger   Group: iGEM10_Washington   (2010-10-18)
Revision as of 22:42, 21 October 2010 by Swanson (Talk | contribs)

Toxin Tse2

A toxic protein originating from Pseudomonas aeruginosa that has been shown to arrest growth in both prokaryotic and eukaryotic cells when expressed intracelluarly. It is a substrate of the Pseudomonas aeruginosa type 6 secretion system. Tse2 has an immunity protein, Tsi2, that, when expressed in conjunction with Tse2 prevents cell death.

Reference showing that Tse2 is toxic, Tsi2 is the antitoxin, and that these proteins are transferred into target Gram-negative organisms via a secretion system: "A type VI secretion system of Pseudomonas aeruginosa targets a toxin to bacteria" [http://www.ncbi.nlm.nih.gov/pubmed/20114026]

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 196
    Illegal NgoMIV site found at 253
    Illegal NgoMIV site found at 378
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/prokaryote/ecoli/nissle
//collections/probiotics
//collections/probiotics/biocontainment
Parameters
None